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SpermPrep TYB Media: Freezing Medium

SpermPrep TYB Media: Freezing Medium

SKU: 90128

Freezing Medium- TYB w/ Glycerol & Gentamicin


Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.


• 2 year shelf life

• Contains:
- 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56°C for 30 minutes
- 12% glycerol
- 10µg/mL gentamicin sulfate

• CE Marked


Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sperm Cryosurvival assay
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• pH
• Osmolality



- 100ml    (Cat# 90128-100)

-20x 5ml   (Cat# 90128-20x5)

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